Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/2895
Title: Release monitoring of single cells on a microfluidic device coupled with fluorescence microscopy and electrochemistry
Authors: Shi, Bao-Xian
Wang, Yu
Lam, Tin-Lun
Huang, Wei-Hua
Zhang, Kai
Leung, Thomas Y. C.
Chan, Helen L. W.
Subjects: Amperometric sensors
Biochemistry
BioMEMS
Cellular biophysics
Electrochemistry
Fluorescence
Microfluidics
Neurophysiology
Optical microscopy
Toxicology
Issue Date: 30-Dec-2010
Publisher: American Institute of Physics
Source: Biomicrofluidics, 30 Dec. 2010, v. 4, no. 4, 043009, p. 1-9.
Abstract: A method for monitoring the biological exocytotic phenomena on a microfluidic system was proposed. A microfluidic device coupled with functionalities of fluorescence imaging and amperometric detection has been developed to enable the real-time monitoring of the exocytotic events. Exocytotic release of single SH-SY5Y neuroblastoma cells was studied. By staining the cells located on integrated microelectrodes with naphthalene-2,3-dicarboxaldehyde, punctuate fluorescence consistent with localization of neurotransmitters stored in vesicles was obtained. The stimulated exocytotic release was successfully observed at the surface of SH-SY5Y cells without refitting the commercial inverted fluorescence microscope. Spatially and temporally resolved exocytotic events from single cells on a microfluidic device were visualized in real time using fluorescence microscopy and were amperometrically recorded by the electrochemical system simultaneously. This coupled technique is simple and is hoped to provide new insights into the mechanisms responsible for the kinetics of exocytosis.
Rights: © 2010 American Institute of Physics. This article may be downloaded for personal use only. Any other use requires prior permission of the author and the American Institute of Physics. The following article appeared in B.X. Shi et al. Biomicrofluidics 4:4, 043009 (2010) and may be found at http://bmf.aip.org/resource/1/biomgb/v4/i4/p043009_s1.
Type: Journal/Magazine Article
URI: http://hdl.handle.net/10397/2895
DOI: 10.1063/1.3491470
ISSN: 1932-1058 (online)
Appears in Collections:MRC Journal/Magazine Articles
AP Journal/Magazine Articles
ABCT Journal/Magazine Articles

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