Please use this identifier to cite or link to this item: http://hdl.handle.net/10397/2535
Title: Telomerase-associated apoptotic events by mushroom Ganoderma lucidum on premalignant human urothelial cells
Authors: Yuen, John W. M.
Gohel, Mayur Danny Indulal
Au, Doris Wai-Ting
Subjects: Ganoderma lucidum
Triterpenes
Bladder cancer
Apoptosis
Telomerase
Prevention
Issue Date: Jan-2008
Publisher: Routledge (Taylor & Francis)
Source: Nutrition and cancer, Jan. 2008, v. 60, no. 1, p. 109-119.
Abstract: The chemopreventive effects of Ganoderma lucidum was tested, using a tumorigenic transformable human urothelial cell (HUCPC) model. These in vitro data show that G. lucidum can inhibit the viability and growth of HUC-PC. This could be explained by a concomitant induction of apoptosis and inhibition of telomerase activity. Significant exteriorization of phosphatidylserine was detected by Annexin-V on cell surface, and the cells subsequently lost membrane integrity for uptake of 7-amino-actinomycin D dye. Additionally, the levels of hydrogen peroxide and 8-hydroxy-2'- deoxyguanosine (8-OHdG) production of the apoptotic cells were significantly increased. The induction of apoptosis and suppression of telomerase activity help to explain the anti-HUC-PC growth properties; however, the induction of oxidative stress requires further study. This study strongly suggests that G. lucidum is a potential source of chemopreventive agents for bladder cancer based on its effectiveness in controlling the premalignant urothelial cell growth and carcinogen-induced transformation.
Rights: © 2008 Taylor & Francis Group, LLC. This is an electronic version of an article published in J.W.M. Yuen, M.D.I. Gohel and D.W.T. Au (2008), Nutrition and Cancer, 60(1), 109-119. Nutrition and Cancer is available online at: http://www.informaworld.com, and the article at: http://www.informaworld.com/smpp/content~db=all~content=a789135069~frm=titlelink
Type: Journal/Magazine Article
URI: http://hdl.handle.net/10397/2535
DOI: 10.1080/01635580701525869
ISSN: 0163-5581 (print)
1532-7914 (online)
Appears in Collections:HTI Journal/Magazine Articles

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