Please use this identifier to cite or link to this item:
Title: Telomerase-associated apoptotic events by mushroom Ganoderma lucidum on premalignant human urothelial cells
Authors: Yuen, John W. M.
Gohel, Mayur Danny Indulal
Au, Doris Wai-Ting
Subjects: Ganoderma lucidum
Bladder cancer
Issue Date: Jan-2008
Publisher: Routledge (Taylor & Francis)
Source: Nutrition and cancer, Jan. 2008, v. 60, no. 1, p. 109-119.
Abstract: The chemopreventive effects of Ganoderma lucidum was tested, using a tumorigenic transformable human urothelial cell (HUCPC) model. These in vitro data show that G. lucidum can inhibit the viability and growth of HUC-PC. This could be explained by a concomitant induction of apoptosis and inhibition of telomerase activity. Significant exteriorization of phosphatidylserine was detected by Annexin-V on cell surface, and the cells subsequently lost membrane integrity for uptake of 7-amino-actinomycin D dye. Additionally, the levels of hydrogen peroxide and 8-hydroxy-2'- deoxyguanosine (8-OHdG) production of the apoptotic cells were significantly increased. The induction of apoptosis and suppression of telomerase activity help to explain the anti-HUC-PC growth properties; however, the induction of oxidative stress requires further study. This study strongly suggests that G. lucidum is a potential source of chemopreventive agents for bladder cancer based on its effectiveness in controlling the premalignant urothelial cell growth and carcinogen-induced transformation.
Rights: © 2008 Taylor & Francis Group, LLC. This is an electronic version of an article published in J.W.M. Yuen, M.D.I. Gohel and D.W.T. Au (2008), Nutrition and Cancer, 60(1), 109-119. Nutrition and Cancer is available online at:, and the article at:
Type: Journal/Magazine Article
DOI: 10.1080/01635580701525869
ISSN: 0163-5581 (print)
1532-7914 (online)
Appears in Collections:HTI Journal/Magazine Articles

Files in This Item:
File Description SizeFormat 
Ganoderma lucidum.pdfPre-published version1.5 MBAdobe PDFView/Open

All items in the PolyU Institutional Repository are protected by copyright, with all rights reserved, unless otherwise indicated. No item in the PolyU IR may be reproduced for commercial or resale purposes.