Methods and agents of decalcifying bone

Abstract

Decalcification can be performed by using acid or other agents. It may be difficult to decalcify large bone block within ours. This invention provides a bone-decalcifying agent including a calcium desolving agent for desolving calcium ions of bones, and a bubble-forming agent for generating microbubbles when the bone-decalcifying agent is exposed to ultrasonic vibration. It is found that bones may be decalcified in a relatively short time using the bone-decalcifying agent of this invention.